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KMID : 0853020120150010039
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Journal of Korean Burn Society
2012 Volume.15 No. 1 p.39 ~ p.44
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The Expression of Human Beta Defensins by Thermal Injury
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Jeong Hyo-Sun
Yun Cheon-Je
Lee Jung-Suk
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Abstract
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Purpose: Defensins are important components of innate immune system. These peptides have antimicrobial activity against a wise variety of pathogens that associated with burn wound infection. In particular, human β-defensins are expressed in normal epidermal region and showed differential expression of some skin disease. We investigated that expression of human β-defensin by in vitro and ex-vivo by thermal condition.
Methods: To investigate the expression of human β-defensins in acute burn condition, we cultured keratinocytes and used to rat’s skin at this experiment. After thermal condition, we showed the expression of β-defensins-2 (hBD-2), -3 (hBD-3), keratins, keratinocyte differentiation and junction protein levels by RT-PCR and immunohistochemistry (IHC).
Results: HBD-2 & involucrin were down-regulated from 1 hr to 8 hrs in mRNA level. But others were not changed in mRNA level. In protein level, hBD-3 was decreased but pan-cytokeratin and β-catenin were not changed.
Conclusion: HBD-2 was down-regulated in thermal injury. Because thermal injury could induce the influence of keratinocyte differentiation and the decrease of skin protection ability. Our results suggested that human β-defensins plays
an important role in protection by several injury. (J Korean Burn Soc 2012;15:39-44)
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KEYWORD
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Thermal injury, Human beta defensins, Involucrin
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